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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Biomaterials. 2018 Feb 13;178:751–766. doi: 10.1016/j.biomaterials.2018.01.032

Figure 5.

Figure 5.

A) Confocal reconstruction of MDA-MB-231 cells invading into HANDBF/MMPx hydrogels with low, medium, and high gradients of EGF555 and Alexa546. B) Normalized invasion distance of MDA-MB-231 cells in low, medium, and high gradients of Alexa546 and EGF555 and non-patterned regions of the HANDBF/MMPx hydrogel (day 6, n=5, mean + standard deviation). EGF555 significantly increased invasion compared to the control Alexa546 gradients (**p<0.01, two-way ANOVA). The slope of the gradient also significantly affected invasion (*p<0.05). Post hoc comparisons are shown graphically. C) Percent of invading MDA-MB-231 cells in low, medium, and high gradients of Alexa546 and EGF555. (day 6, n=5, mean + standard deviation). D) Normalized cell number of MDA-MB-231 cells in low, medium, and high gradients of Alexa546 and EGF555 and non-patterned regions of the HANDBF/MMPx hydrogel (day 6, n=5, mean + standard deviation).