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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Biomaterials. 2018 Feb 13;178:751–766. doi: 10.1016/j.biomaterials.2018.01.032

Figure 7.

Figure 7.

A) Confocal reconstruction of MCF-7 cells cultured on HANDBF/MMPx hydrogels with medium gradients of EGF555 and Alexa546. Side views are zoomed in on a portion of the gradient so the MCF-7 cells can be clearly visualized as a cluster on the surface of the hydrogel. B) MCF-7 cells failed to invade into medium Alexa546 and EGF555 gradients after 6 days of culture (n=6). C) Normalized cell number of MCF-7 cells in medium gradients of Alexa546 and EGF555 and non-patterned regions of the HANDBF/MMPx hydrogel (day 6, n=6, mean + standard deviation). EGF555 had no significant influence on MCF-7 cell number (two-way ANOVA).