Table 1. Typing schemes for TPA bacteria (since 1998) and the new proposed MLST scheme.
| Typing method | Genes/Loci | Methods | Year of introduction | References |
|---|---|---|---|---|
| CDCa -typing (CDCT) | arp, tprE, tprG, tprJ | RFLPb of tpr genes, determination of repeats in arp gene | 1998 | [11] |
| Sequencing-based molecular typing (SBMT) | TP0136, TP0548, 23S rRNA | Sanger sequencing | 2006 | [12] |
| Enhanced CDCa -typing (ECDCT) | arp, tprE, tprG, tprJ, TP0548 | RFLPb of tpr genes, determination of repeats in arp gene, Sanger sequencing of TP0548 | 2010 | [13] |
| CDCa -typing with additional analyses of rpsA gene (CDC-rpsA) | arp, rpsA, tprE, tprG, tprJ | RFLPb of tpr genes, determination of repeats in arp and rpsA genes | 2010 | [14] |
| Proposed MLST | TP0136, TP0548, TP0705, 23S rRNA | Sanger sequencing | 2018 | This study |
a Centers for Disease Control and Prevention, Atlanta, United States.
bRFLP—restriction fragment length polymorphism.