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. 2018 Jul 30;13(7):e0201432. doi: 10.1371/journal.pone.0201432

Fig 3. Immunodetection of GART and PPAT in CR-HGPRT cells.

Fig 3

Non-transfected CR-HGPRT cells exhibited endogenous GART (B, K) and PPAT (A, J) proteins in the form of fine granules, and their fluorescent signals showed a high degree of overlap in both the purine-depleted medium (C) and the purine-rich medium (L). In cells transfected with a vector encoding the wt HGPRT protein, the endogenous proteins formed fine granules with high signal colocalization in the purine-depleted medium (D, F), whereas in the purine-rich medium, the proteins remained diffuse (M, N) with no colocalization (O). The values of the fluorescent signal overlaps are shown in pseudocolour, and the scale is shown at the lower right in the corresponding LUT.