Fig 5. Immunodetection of PPAT and GART in CR-PFAS cells transfected with constructs encoding wt-PFAS proteins.

In cells transfected with constructs encoding wt-PFAS protein, the endogenous proteins PPAT (A) and GART (B) were observed in the form of fine granules with their fluorescent signals showing a high degree of overlap in purine-depleted medium (C), whereas in purine-rich medium, the proteins PPAT (G) and GART (H) remained diffuse and did not colocalize (I). The same behaviour was observed in the control HeLa cells (Fig 9). When the cells were not transfected, the endogenous proteins remained diffuse regardless of the level of purines in the media (D, E, J, K) and did not colocalize (F, L). The values of the fluorescent signal overlaps are shown in pseudocolour, and the scale is shown at the lower right in the corresponding LUT.