Table 1. Cell lines used in this study.

Cell line	Derived from:	Genotype	Description
WT/WTγ	EATRO 1125 AnTat 1.1 90:13	ATPγ/Δatpγ::atpγWT+PURO	One F1FO-ATP synthase subunit γ allele replaced with a WT copy and the puromycin resistance gene (PURO)
WT/L262Pγ #1	EATRO 1125 AnTat 1.1 90:13	ATPγ/Δatpγ::atpγL262P+PURO	One F1FO-ATP synthase subunit γ allele replaced with a copy with the L262P mutation and the puromycin resistance gene (PURO)
WT/L262Pγ #2	EATRO 1125 AnTat 1.1 90:13	ATPγ/Δatpγ::atpγL262P+PURO	One F1FO-ATP synthase subunit γ allele replaced with a copy with the L262P mutation and the puromycin resistance gene (PURO)
WT/L262Pγ (kDNA0) #1	WT/L262Pγ #1	ATPγ/Δatpγ::atpγL262P+PURO	Cell line WT/L262Pγ #1 induced to lose kDNA through exposure to acriflavine
WT/L262Pγ (kDNA0) #2	WT/L262Pγ #2	ATPγ/Δatpγ::atpγL262P+PURO	Cell line WT/L262Pγ #2 induced to lose kDNA through exposure to acriflavine
WT/L262Pγ (kDNA0) #3	WT/L262Pγ #3	ATPγ/Δatpγ::atpγL262P+BSD	Cell line WT/L262Pγ #3 after spontaneous loss of kDNA