Figure 7.

PTAP motif duplication confers higher levels of inducibility on HIV-1C. HEK293T cells were cotransfected with 200 ng of one of the three viral vectors and progressively increasing concentrations of the Tsg101–FLAG expression vector ranging from 200 to 2,000 ng. The secretion of p24 into the medium was quantitated at 48 h and normalized against the β-gal transfection control. Data presented are representative of two independent experiments performed in triplicate wells. Statistical analysis was performed using the two-way ANOVA test.