Table 2.

Kinetic data of AxMan113A toward manno-oligosaccharides

The kinetic parameters were determined at 45 °C for 5 min in 50 mm McIlvaine buffer (pH 6.5).

Substrate	Vmax	Km	kcat	kcat/Km
	μmol min−1 mg−1	mm	s−1	s−1 mm−1
M2	162.2 ± 7.3	74.3 ± 4.4	0.098	0.0013
M3	1413.8 ± 65	29.1 ± 2.3	0.853	0.029
M4	1622.5 ± 86	12.7 ± 1.1	0.979	0.077
M5	1764.7 ± 94	5.3 ± 0.4	1.065	0.201
M6	2500 ± 152	3.9 ± 0.3	1.508	0.387