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. 2018 Jun 6;293(30):11878–11890. doi: 10.1074/jbc.RA117.000194

Figure 5.

Figure 5.

BmNPV infection activates BmRelish through the cGAMP-BmSTING pathway. A, cGAMP (3 μg/ml) and BmNPV virus were delivered to BmE cells overexpressing BmRelish together with BmSTING or BmRelish alone, and BmRelish protein was then measured by Western blotting. All Western blotting signals were analyzed, and densitometric analysis of the BmRelishact protein bands from the Western blots was performed using ImageJ software. B, confocal analysis of BmE cells overexpressing BmRelish alone or together with BmSTING and treated with cGAMP (cGAMP) or only the same transfection reagent (Mock) (scale bar, 10 μm). C, relative viral DNA level in BmE cells overexpressing both BmRelish and BmSTING, BmRelish, or BmRelishact alone and infected with BmNPV at an MOI of 1 was determined by qPCR at 72 hpi. D, fluorescence microscopy (GFP) of BmE cells overexpressing both BmRelish and BmSTING, BmRelish, or BmRelishact alone and infected with BmNPV at 72 hpi (scale bar, 100 μm). E–H, expression of BmCecB (E), BmCecA (F), BmGloverin (G), and BmLysozyme (H) in BmE cells transfected with BmSTING and BmRelish separately or together. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant. Error bars, S.D.