Figure 2.

Transcriptional profiling of lung CD4⁺ T-cells. A, principal component analysis of 3932 differentially expressed genes identified from a generalized linear model and ANOVA-like testing with FDR q-value ≤ 0.05. Ellipses represent normal contour lines with one S.D. probability. B, Pearson correlation distance clustering. C, k-means clustering of differentially expressed genes with k = 4 and scaled as Z-score across rows. D, top five gene ontology (GO) processes derived from each k-means cluster ranked by −log₁₀-transformed FDR q-value. Data are from three replicates per group, each representing pooled cells from three neonatal and two juvenile mice per replicate. ER, endoplasmic reticulum.