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. 2018 Jun 13;293(30):11837–11849. doi: 10.1074/jbc.RA118.002633

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© 2018 Cruz et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 3. — β2AR agonists reduce DUX4 expression in FSHD myotubes. A, luciferase activity measured in ZSCAN4 RGA–transduced FSHD1 (FSHD940) myotubes treated with formoterol (FOR) and albuterol (ALB). B, qPCR for ZSCAN4 (top) and TRIM43 (bottom) in FSHD940 myotubes treated with varying concentrations of formoterol (FOR) and albuterol (ALB). C, qPCR for DUX4 in FSHD940 myotubes treated with 100 nm formoterol (FOR) and albuterol (ALB); *, p < 0.05. D, quantification of MYHC-positive nuclei by high content imaging (percentage of DMSO control). Myotubes were treated with 100 nm formoterol (FOR) or albuterol (ALB) for 48 h. Error bars, S.E.