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The cAMP analog 8-Br-cAMP reduces DUX4 expression in FSHD myotubes. A, luciferase activity measured in ZSCAN4 RGA–transduced FSHD1 (FSHD940) myotubes treated with 8-Br-cAMP. B, qPCR for ZSCAN4 (top) and TRIM43 (bottom) in FSHD940 myotubes treated with varying concentrations of 8-Br-cAMP. C, qPCR for DUX4 in FSHD940 myotubes treated with 500 μm 8-Br-cAMP; *, p < 0.05. Error bars, S.E.
