Figure 1.

Cardiac-specific deletion of Jarid2 using Nkx2.5-Cre mice (Jarid2^Nkx). A, genomic DNAs were isolated from the tail (T), heart (H), and brain (B) at E18.5. PCR was performed using primers located outside two loxP sites containing exon3 of Jarid2 (61), yielding floxed allele (1054 bp) or floxed-out allele (354 bp). B, Jarid2 mRNA levels were detected by qRT-PCR relative to 18S RNA on the control or Jarid2^Nkx heart, brain, or liver at E18.5, n = 3. *, p ≤ 0.05. C, Western blotting was performed with Jarid2 antibody on control or Jarid2^Nkx hearts at E13.5. GAPDH is a loading control. D, immunostaining analysis was performed on comparable transverse heart sections from E10.5 control (a–d) or Jarid2^Nkx (e–h) embryos using Jarid2 (red) and MF20 (green) antibodies. Arrows indicate the endocardium and arrowheads indicate the myocardium. Scale bar, 50 μm.