Figure 5.
Jarid2 occupies a specific region at the Isl1 locus. A, VISTA alignment was performed at the Isl1 genomic locus spanning about 70 kb for mouse, monkey, human, and rat to determine conserved regions. Here, the promoter region around the 10–kb region near the transcription start site (arrow) was analyzed. Gray bars indicate regions with greater than 75% conservation, and black bars indicate exons. Arrowheads indicate primer sites. B, Jarid2 occupancy at the conserved regions was measured by qChIP assays on Jarid2Nkx or control hearts using Jarid2 antibody. The bars show enrichment compared with each input. C, qChIP assays were performed on control or Jarid2Nkx hearts at the −0.5–kb region of Isl1 using Jarid2, Ezh2, H3K27me3, or H3K4me3 antibody, n = 3 (*, p ≤ 0.05; **, p ≤ 0.01).