Figure 5.

Activation of Op vinculin binding to F-actin by Op talin. A and B, Op vinculin co-sedimentation with F-actin in the absence (A) or presence (B) of the Op talin peptide. Op vinculin was added at increasing concentrations to 1.5 μm F-actin. Background Op vinculin pelleting in the absence of F-actin is shown in the lower panels. Op talin peptide was used in 5-fold excess to Op vinculin. The assay was performed in duplicate. C, bound Op vinculin/actin plotted against the concentration of free Op vinculin concentration is shown for binding without (triangles) or with (circles and squares) the Op talin peptide.