Skip to main content
. 2018 Jul 17;7:e38752. doi: 10.7554/eLife.38752

Figure 3. Fic-mediated AMPylation of BiP is required for photoreceptor maintenance.

(A) A representative ERG trace in response to a 1 s light pulse displaying the sustained negative potential (SNP), representing the depolarization within photoreceptor neurons, and the ON and OFF transients, reflecting post-synaptic activity of lamina neurons. (B) Representation of the different light treatments of flies before ERG recordings: 3 days of 12 hr light (500 lux) and 12 hr dark (LD), 3 days of continuous light (LL) or 3 days of continuous light followed by 3 days of LD (Rec). 1 s light pulses were performed at 4 s intervals. (C) Representative traces from w1118, fic30C, BiPWT and BiPT366A flies. Under LL, fic30C and BiPT366A mutants lose ON and OFF transients (red arrows) and have reduced SNPs. The changes are reversed after 3 days of recovery (Rec). (D) Quantification of key components of ERGs shown in panel C. Bar graphs show means ± SD. ****p<0.0001; ***p<0.001; **p<0.01; *p<0.05; n = 24 flies for each genotype/condition, pooled from three independent biological replicas.

Figure 3—source data 1. Relates to Figure 3D.
Quantification of ERG components with LD, LL, and Recovery.
elife-38752-fig3-data1.xlsx (56.9KB, xlsx)
DOI: 10.7554/eLife.38752.015

Figure 3.

Figure 3—figure supplement 1. Light-induced defects in light-startle activity in fic30C mutants.

Figure 3—figure supplement 1.

(A, B, and C) Actogram of w1118 or fic30C flies reared in LD for three days (A), LL for three days (B), or recovery condition (three days in LL then three days in LD) (C). Light pulse is indicated by upper bars. Data is averaged from three biological replicas, each containing 16 flies per genotype. Data were collected in two-minute bins. Error bars represent Standard Error. (D) Quantification of change in beam breaks per 2 min bin for the 10 min intervals before and after the onset of the light pulse in each experiment. Bar graphs show means ± SD. ***p<0.01, n = 3 experimental repeats with 16 flies per genotype and condition. Dead flies and those with a change in activity greater than three deviations from the median were excluded.
Figure 3—figure supplement 1—source data 1. Relates to Figure 3—figure supplement 1A, B, C and D.
Quantification of light-induced startle behavior- average activity and delta calculations.
elife-38752-fig3-figsupp1-data1.xlsx (17.2KB, xlsx)
DOI: 10.7554/eLife.38752.016
Figure 3—figure supplement 2. fic30C mutants are sensitive to constant light, regardless of total intensity.

Figure 3—figure supplement 2.

(A) Representative ERGs of flies following 3 days of LL or LD with either 500 or 5000 lux light, showing fic30C null animals lose ON/OFF transients and have reduced SNPs with constant light, regardless of intensity, but under LD conditions, even at 5000 lux, have healthy ERG responses. (B) Quantification of ERG data. Bar graphs show means ± SD. ****p<0.0001; ***p<0.001; *p<0.05; n = 10 flies per genotype and condition.
Figure 3—figure supplement 2—source data 2. Relates to Figure 3—figure supplement 2B.
Quantification of ERG components with 500 and 5000 lux.
elife-38752-fig3-figsupp2-data2.xlsx (49.6KB, xlsx)
DOI: 10.7554/eLife.38752.017
Figure 3—figure supplement 3. Fic30C mutants recover ERG properties in 72 hours after 10 days of LL.

Figure 3—figure supplement 3.

(A) Representative ERGs of flies following 10 days of LL, 10 days LD (500 lux), and 3 days Recovery following 10 days. (B) Quantification of ERG data. Bar graphs show means ± SD. ****p<0.0001; ***p<0.001; *p<0.05; n = 8 flies per genotype and condition.
Figure 3—figure supplement 3—source data 3. Relates to Figure 3—figure supplement 3B.
Quantification of ERG components with 10 days of LL.
elife-38752-fig3-figsupp3-data3.xlsx (43.4KB, xlsx)
DOI: 10.7554/eLife.38752.018