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. Author manuscript; available in PMC: 2019 Mar 16.
Published in final edited form as: Science. 2018 Mar 16;359(6381):1247–1250. doi: 10.1126/science.aao6595

Fig. 3.

Fig. 3.

A) 35 GHz CW 13C ENDOR spectra for intermediate I with (methionine-13C5)-SAM. B) Isotope EPR study of the organic radical intermediate II on EF2 quenched at 2 min (T=70 K). C) Cluster and bound SAM in the structure of CmnDph2 (left), in comparison with cluster and bound SAM in the structure of PFL-AE (right, PDB 3CB8) (13). Distances are given in angstroms. The black and brown asterisks label Cγ,Met of SAM and the differentiated iron of the [4Fe-4S] cluster, respectively. D) Overlay of SAM and cluster in CmnDph2 and PFL-AE structures. E) CmnDph2 active site showing the binding of SAM. Hydrophobic interactions with purine ring of SAM are not shown for clarity.