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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Appl Physiol Nutr Metab. 2018 Mar 8;43(8):795–805. doi: 10.1139/apnm-2017-0858

Figure 1.

Figure 1

Isolated epitrochlearis muscle strips were incubated ex vivo in four steps. During Step 1, each muscle strip from a single animal was incubated with No serum or AICAR (in Krebs-Henseleit Buffer; KHB) with 8 mM glucose, serum alone, KHB with 8 mM glucose and 2 mM AICAR, or serum and 2 mM AICAR for 60 min. During Step 2, muscle strips were incubated in KHB with 8 mM glucose for 180 min. During Step 3, all four muscle strips from 10 rats were incubated in KHB with No Insulin, and all four strips from the remaining 10 rats were incubated with 1.2 nM Insulin for 30 minutes. During Step 4, all muscle strips were incubated in KHB with 2 mM mannitol and radiolabeled 3-O-methyl-D-glucose (3-MG) and the same insulin concentration as Step 3 for 20 minutes.