Figure 10.

HKII in epitrochlearis muscles. A) For muscles incubated without insulin, RM 2-way ANOVA indicated a main effect of serum* (p=0.001). Post hoc analysis revealed depressed HKII with prior serum < No AICAR or serum^a (p<0.05) and prior serum+AICAR < AICAR alone^d (p<0.01). No effects were observed in muscles incubated with insulin. B) Two-way ANOVA did not indicate any main effect of AICAR or insulin on HKII expression. All data are shown as mean±SEM in the graph with representative blots above the bars and loading control below the bottom graph (n=10/group). MemCode, a protein stain used to confirm efficacy of transfer before the blocking step, was used as a loading control by quantitating the band at molecular weight 45 kD (Antharavally et al. 2004; Castorena et al. 2014).