Fig. 11.

Influences of GMF on the apoptotic and anti-apoptotic markers expression. Cells were treatedin the presence orabsence of GMF (100 ng/ml) and/or MPP⁺ (300 μm) for 24 h. After the incubation period cell lysates from these cells are used to detect the apoptotic and anti-apoptotic markers expressions by western blot Exposure of N27 cells to GMF significantly increased the cytosolic cytochrome-c (A), increased Bax, reduced Bcl2 (B) and increased caspases 3, 7, 8 and 9; (C) expression when compared with control cells. The band density was quantified by densitometry. β-actin was used as internal standard to normalize the intensity of the protein expressions. Values are given as mean ± SD. of four experiments in each group. ^*p< 0.05 compared to control, and ^*p< 0.05 compared to GMF treated group.