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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Mol Neurobiol. 2018 Jan 30;55(9):7132–7152. doi: 10.1007/s12035-018-0882-6

Fig. 3.

Fig. 3

Effect of GMF on ROS expression in dopaminergic N27 cells. N27 cells were seeded (3×106) in 96 well plate and incubated with GMF (100 ng/ml) and/or MPP+ (300 μm) for 24 h. After the incubation period cells washed with PBS and stained with DCFDA green flourescent dye for 35 mins. Microphotographs showing the toxic putative effect of GMF induced ROS generation (green flourescence) by DCFDA staining (200X). The optimum dose of GMF treatment significantly increased the levels of ROS (similar to MPP+ treated cells) as compared to control cells. Incubation of cells with both GMF and MPP+ drastically increased ROS generation compared with cells incubated with only GMF or MPP+. Values are given as mean ± SD. of four experiments in each group. *p< 0.05 compared to control, and *p< 0.05 compared to GMF treated group.