Fig. 4.

Effect of GMF on apoptotic morphological changes in dopaminergic N27 cells. N27 cells were seeded (3×10⁶) in 96 well plate and incubated with GMF (100 ng/ml) and/or MPP⁺ (300 μm) for 24 h. After the incubation period the cells werewashed with PBS and stained with EtBr/AO fluorescent dye for 15 mins. Images were taken using fluorescence microscope at 200X. Photomicrographs show that GMF exposure increased apoptotic cell death of dopaminergic cells (A). The percentage of viable cells were measured after termination of incubation period (B). The bright green color indicate control cells, orange color indicates the apoptotic cell death (B). ^*p< 0.05 compared to control, and ^*p< 0.05 compared to GMF treated group.