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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Mol Neurobiol. 2018 Jan 30;55(9):7132–7152. doi: 10.1007/s12035-018-0882-6

Fig. 7.

Fig. 7

Effect of GMF exposure on intracellular mitochondrial morphology detected by MitoTracker Red CMXRos fluorescent staining method. N27 cells were seeded (3×106) in 6 well plate and incubated with GMF (100 ng/ml) and/or MPP+ (300 μm) for 24 h under standard conditions. After incubation period cells washed with PBS. 1 mM of MitoTracker Red CMXRos and 16 mM Hoechst fluorescent dye were prepared and incubated with cells for 20 mins. Exposure of GMF to dopaminergic N27 cells directly affects the cytoplasmic mitochondria mass and induced mitochondrial morphological changes compared with control cells. GMF treatment reduced the MitoTracker Red CMXRos fluorescent stained mitochondria mass as compared with control cells. Images were taken using fluorescence microscopy at 200X.