Fig. 8.

Effect of GMF on PGC-1α expression. N27 cells were seeded in T25 cell culture flask and incubated with GMF (100 ng/ml) and MPP⁺ (300 μm) for 24 h under standard conditions. After the incubation period cells were washed with PBS and cell lysates were prepared from these cells for western blot studies. GMF treatment significantly reduced expression of PGC-1α expression as seen by immunoblotting as compared with control cells (A). β-actin was used as an internal protein control to show equal protein loading (B). Western blot bands were quantified and the values are expressed as arbitrary units and given as mean ± SD of four experiments in each group. *p< 0.05 compared to control