Table 1.
Sequences of primers used in this study
| Primer | Sequence (5′ to 3′) | Purpose | Size (bp) |
|---|---|---|---|
| LB047-1 | F: GCGCATATG(Nde I)AAACGATTTTGTTTG | Detection and expression of LB047 gene | 1317 |
| R: GCGCTCGAG(Xho I)TTTACAACCTTGCATTTC | |||
| LB047-2 | F: ATGAAACAACTTTGTTTA | Detection of LB047 gene | 1323 |
| R: CTTACAAGGAGGACTTTC | |||
| LB047-3 | F: TTCTGCGTGACACTCCTTAC | Detection of LB047 mRNA | 156 |
| R: CTTCTTCGTCCATCAAACCT | |||
| 16S-RNA | F: CTTTCGTGCCTCAGCGTCAGT | Inner reference in LB047 mRNA qRT-PCR | 145 |
| R: CGCAGCCTGCACTTGAAACTA | |||
| Ms-Fas | F: TGTGAACATGGAACCCTTGA | Detection of J774A.1 Fas mRNA | 177 |
| R: TTCAGGGTCATCCTGTCTCC | |||
| Ms-FasL | F: CATCACAACCACTCCCACTG | Detection of J774A.1 FasL mRNA | 162 |
| R: GTTCTGCCAGTTCCTTCTGC | |||
| Ms-actin | F: AGAGCGGGCCTTGAGAAAAG | Inner reference in J774A.1 Fas/FasL mRNA qRT-PCR | 111 |
| R: TGGAGAGCCTGGATTGTCATC | |||
| Hu-Fas | F: AGCTTGGTCTAGAGTGAAAA | Detection of THP-1 Fas mRNA | 180 |
| R: GAGGCAGAATCATGAGATAT | |||
| Hu-FasL | F: CACTTTGGGATTCTTTCCAT | Detection of THP-1 FasL mRNA | 160 |
| R: GTGAGTTGAGGAGCTACAGA | |||
| Hu-actin | F: ATAGCACAGCCTGGATAGCAACGTAC | Inner reference in THP-1 Fas/FasL mRNA qRT-PCR | 158 |
| R: CACCTTCTACAATGAGCTGCGTGTG |
Underlined areas indicate the sites of endonucleases
F forward primer, R reverse primer, Ms mouse, Hu human