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. 2018 Jul 24;9:1723. doi: 10.3389/fimmu.2018.01723

Figure 1.

Figure 1

Phagocytosis of zymosan particles in RAW 264.7 and plasmalogen-deficient RAW.108 cells. The cells were exposed to fluorescent non-opsonized (Zym) or opsonized zymosan (OpZ) particles for 30 min, as indicated (10 particles per cell, equivalent to approximately 0.2 mg per million cells), and analyzed for phagocytosis of fluorescent zymosan particles by confocal microscopy (red color, middle columns). DAPI (1 µg/ml) was used to mark the nuclei (blue, left columns). Nomarski and merge images are also shown (right columns). The average of three independent experiments with 20 determinations each is shown (mean ± SEM) (bottom panel). Original magnification, ×20. **Significantly different (p < 0.01) from RAW 264.7 cells exposed to OpZ.