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. 2018 Jul 24;9:1723. doi: 10.3389/fimmu.2018.01723

Figure 4.

Figure 4

Effect of lysoPlsEtn on the phagocytosis of opsonized zymosan by RAW 264.7 and plasmalogen-deficient RAW.108 cells. The cells were either untreated (brown bars) or treated with 10 µM lysoPlsEtn (orange bars) or 10 µM lysoPtdEtn (yellow bars) for 10 min. Afterward, fluorescent zymosan particles (10 particles per cell) were added for 30 min, and phagocytosis was analyzed by confocal microscopy (red color, middle columns). DAPI (1 µg/ml) was used to mark the nuclei (blue; left columns). Nomarski images are also shown (right columns). The average of three independent experiments with 20 determinations each is shown in the bottom panel (mean ± SEM). Phagocytic indexes in the bottom panel are given as percent values with respect to controls without added lysophospholipids to allow for direct comparison between the responses of RAW 264.7 cells versus RAW.108 cells. Actual values were 112 ± 4 and 49 ± 2 for RAW 264.7 cells and RAW.108 cells, respectively. Original magnification, ×20. **Significantly different (p < 0.01) from untreated cells.