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Effect of lysophospholipids on zymosan-induced signaling. RAW.108 cells were incubated with 10 µM lysoPtdEtn, 10 µM lysoPlsEtn or neither, as indicated, for 10 min. Afterward, the cells were stimulated with opsonized zymosan (OpZ) for the indicated times, and the phosphorylation of extracellular-regulated kinases (ERK) p44 and p42 was studied by immunoblot. The panel below the blots shows the relative quantification of p44 and p42 phosphorylation with respect to total ERK.
