FIGURE 3.

pSCBe expression vector generation. (A) pSCBe-MCS fragment synthesized from IDT. The fragment contains two 26 bp upstream and downstream overlap sequences for Gibson assembly; Trc1O promoter which has lac operator; RBS site; an N-terminal FLAG tag; MCS with 10 unique enzyme digestion sites when clone into NotI and SpeI digested pSCB-YFP; and a C-terminal His tag. Number 6267 to 6533 indicated fragment position on pSCBe vector. (B) Expression vector pSCBe graph. The detail generation procedure and property of plasmid was described in “Materials and Methods” and “Results” sections.