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. 2018 Jul 24;9:1633. doi: 10.3389/fimmu.2018.01633

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Copyright © 2018 Li, Gong, Tai, Li, Wang, Zhao, Zhang, Yang, Yang, Li and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of proteins in Neospora caninum extracellular vesicles (EVs). (A) Top, silver staining of the total proteins and EVs of N. caninum. Bottom, western blotting analyses of 14-3-3, enolase, and HSP70 in the purified EVs. (B) Gene ontology annotations for all 705 proteins in the N. caninum EVs. (C) Western blot analysis of the EVs using anti-N. caninum mouse serum. M, total crude proteins of N. caninum; E, total proteins of the N. caninum EVs.