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. 2018 Jul 24;9:1642. doi: 10.3389/fimmu.2018.01642

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Copyright © 2018 Waldner, Zhang, James, Allbright, Havis, Bliley, Almadori, Schweizer, Plock, Washington, Gorantla, Solari, Marra and Rubin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The comparison of immunomodulating potency between autologous and allogenic s.c.ASC (A) revealed no statistical significance. Three biological replicates (n = 3) with three different donor/recipient combinations were analyzed. All cells demonstrated a dose-dependent suppression in a tritiated thymidine assay. Panel (B) shows a representative carboxyfluorescein succinimidyl ester mixed lymphocyte reaction stimulated by allogenic splenocytes: allogeneic (ASCsyn) ASC showed similar suppressive potential if compared with allogeneic ASC (ASCallo) or bone marrow-derived mesenchymal stem cells on CD3+, CD4+ cells (B).