Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 24;9:1642. doi: 10.3389/fimmu.2018.01642

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Waldner, Zhang, James, Allbright, Havis, Bliley, Almadori, Schweizer, Plock, Washington, Gorantla, Solari, Marra and Rubin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Head to head comparison between trans-well and standard well proliferation assay stimulated with phytohemagglutinin (PHA). Allogenic mesenchymal stem cell (MSC) deriving from the same donor were added in different ratios to carboxyfluorescein succinimidyl ester stained responder cells, resulting in a dose-dependent suppression of CD3+, CD4+ cells. Absence of cell contact (A) did not result in a loss of suppression performance compared with standard co-culture (B). When adipose-derived mesenchymal stem cells (ASCs) and bone marrow-derived mesenchymal stem cells (BMSCs) were mixed and added to co-cultures (C) at a constant ratio of 4:1 between responder peripheral blood mononuclear cells and the MSCs added, a synergistic effect of s.c.ASC and BMSC is demonstrated resulting in a significant suppression (p < 0.01), independent on the composition of the mixed MSC (more ASC vs. more BMSC). Results for mixed MSCs showed no significant difference if compared with single-cell types.