Table 1.
Semi-quantitation of anterogradely labeled axons in representative terminal regions following injections of biotin dextran amine (BDA) in the caudal nucleus of the tractus solitarius (cNTS) at the level of the area postrema (AP), depending on size and density of tracer deposits of each injection site.
| Specimen type | Animal number | PBL | LC | PAG | PVH | ACe | BNST |
|---|---|---|---|---|---|---|---|
| Case 1a* | |||||||
| (#1–4) | 4 | +++ | ++ | ++ | ++ | + | ++ |
| Case 1b | |||||||
| (#1, 2) | 2 | ++ | + | + | + | − | − |
| Case 2* | |||||||
| (#1–3) | 3 | + | − | − | − | − | − |
| Case 3a | |||||||
| (#1) | 1 | + | − | + | − | − | − |
| Case 3b | |||||||
| (#1, 2) | 2 | + | + | − | − | − | − |
| Case 3c* | |||||||
| (#1, 2) | 2 | + | + | + | − | − | − |
| Case 3d | |||||||
| (#1) | 1 | − | + | − | − | − | − |
| Case 3e | |||||||
| (#1) | 1 | − | + | + | − | − | − |
*Axon terminals in lateral parabrachial nucleus (PBL) of each case are shown in Figure 4. Based on the visual examination of BDA axonal labeling in each brain region of tissue sections: “−” extremely few or no potential boutons or varicose axons; “+” lightly scattered boutons and/or varicose axons, “++” moderately dense boutons and varicose axon, “+++” dense axon terminal field with abundant branches and boutons.