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. 2018 Jul 24;5:90. doi: 10.3389/fcvm.2018.00090

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Copyright © 2018 Puttini, Plaisance, Barile, Cervio, Milano, Marcato, Pedrazzini and Vassalli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of pluripotency-associated genes (Oct4, Nanog) and cardiac-specific transcription factors (Nkx2.5, Mef2c, GATA4, Tbx5). (A) Quantitative RT-PCR analyses of freshly isolated atrial cells (n = 5/group). (B) Outgrowth cells (n = 5/group). 2^−ΔΔCt values for ALDH^hi vs. ALDH^lo cells are shown. (C). Freshly isolated ALDH^hi cells. Immunostaining for Nkx2.5 (red), GATA4 (green), and Mef2c (green); DAPI nuclear counterstaining (blue). Nkx2.5/GATA4 and Nkx2.5/Mef2c double stainings are shown.