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. 2018 Jul 24;5:90. doi: 10.3389/fcvm.2018.00090

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Copyright © 2018 Puttini, Plaisance, Barile, Cervio, Milano, Marcato, Pedrazzini and Vassalli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cardiac differentiation of ALDH^hi and ALDH^lo atrial cells using a Notch modulation-based protocol. (A) real-time RT-PCR expression analyses of cardiac-specific (Myh6, Myh7) and smooth muscle-specific genes (Myh11) under expansion (Exp) and differentiation conditions, with or without Notch modulation. 2^−ΔCtvalues are shown. One example of two independent experiments performed in triplicates is shown; *p < 0.05 vs. cells in expansion or between the indicated conditions. (B) Immunostaining of ALDH^hi and ALDH^lo cells for smooth musche-myosin heavy chain (smMHC) and cardiac-specific α-actinin, with or without Notch modulation. (C) Immunostaining of ALDH^hi and ALDH^lo cells for cardiac-specific α-actinin and troponin I (cTNNI) under Notch-modulated conditions. (D) Quantitative analyses of smMHC, α-actinin, and α-actinin/cTNNI positive cells; *p < 0.05 vs. cells in expansion (a minimum of 2000 cells in 30 different fields at 40x magnification were analyzed for quantification).