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. 2018 Jul 24;5:90. doi: 10.3389/fcvm.2018.00090

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Copyright © 2018 Puttini, Plaisance, Barile, Cervio, Milano, Marcato, Pedrazzini and Vassalli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RT-PCR analysis of ALDH isoform gene expression in different populations. (A) ALDH^hi cells isolated using enzymatic techniques. Upper panel: ALDH isoform expression (2^−ΔCt values; n = 4 patients). Lower panel: Relative expression in ALDH^hi vs. ALDH^lo cells (2^−ΔΔCt values; n = 4). (B) Western blot showing ALDH1A3 protein expression in unsorted, ALDH^hi and ALDH^lo atrial cells. (C) Outgrowth cells. Relative expression in ALDH^hi vs. ALDH^lo cells is shown (n = 3). (D) Total atrial tissue (2^−ΔCt values; n = 3). ND, not determined (2^−ΔΔCt values that could not be calculated).