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. 2018 Jul 24;5:90. doi: 10.3389/fcvm.2018.00090

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Copyright © 2018 Puttini, Plaisance, Barile, Cervio, Milano, Marcato, Pedrazzini and Vassalli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ALDH isoform-specific loss- and gain-of-function studies. (A) Flow cytometry plots of ALDH^hi sorted, Aldefluor-reacted cells (with or w/o DEAB) after siALDH1A3 transfection or mock transfection (no siRNA). (B) siALDH1A3 dose-response study. ALDH^hi cell numbers after siALDH1A3 transfection using the indicated concentrations relative no siRNA. (C) ALDH^hi cell numbers after siALDH-isoform-specific transfection (10 nM) relative to no siRNA). (D) EdU⁺ cell numbers (%) after siALDH1A3 transfection (10 nM) relative no siRNA. (E) Flow cytometry plots of ALDH^lo sorted, Aldefluor-reacted cells (with or w/o DEAB) transduced with pMSCVpuro-ALDH1A1 or pMSCVpuro-ALDH1A3 retroviral vectors, or control vector with no insert (pMSCVpuro). (F) ALDH^hi cell numbers after transduction with pMSCVpuro-ALDH1A1 or pMSCVpuro-ALDH1A3 relative to control vector. (G) EdU⁺ cell numbers (%) after transduction with pMSCVpuro-ALDH1A1 or pMSCVpuro-ALDH1A3 relative to control vector; *p < 0.05.