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. 2018 Jul 24;12:218. doi: 10.3389/fncel.2018.00218

Figure 5.

Figure 5

Only 100 μM OAG increased the phasic pheromone response while all other concentrations of both DAG-analogs caused reductions. (A,C) Box-plots show the phasic pheromone response [=frequency of the first 6 bombykal-dependent action potentials (APs)] over the 2 h recordings, with or without (control) infusion of different concentrations of DOG or OAG with 0.1% DMSO. A significant decrease in the phasic pheromone response was found for all concentrations of DOG (1, 100, 200 μM) as well as for 1 and 200 μM OAG at both Zeitgebertimes. Only application of 100 μM OAG increased the phasic pheromone response significantly during the late activity phase and at rest. (B,D) The percentage change illustrates that the highest concentrations of either DAG-analog resulted in the strongest reduction of the AP frequencies in comparison to the controls. Solely 100 μM OAG elevated the frequencies by about 30% in the late activity phase as well as during rest (for mean values ± SEM see S4; for exact P-values see S5; n.s. = not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; Wilcoxon test, Mann-Whitney test or Kruskal-Wallis test with Dunn's post-hoc test for multiple comparison).