Table 2.

CD4 and CD8 naïve and memory cell sorting.

Donor	Age	Group	Subset	Replica	Number of sorted cells	Number of CDR3 reads	Number of CDR3 clonotypes
Donor 1	25	Young	CD4	1	500,000	528,824	38,614
				2	500,000	475,392	240,80
			CD8	1	500,000	564,227	26,988
				2	500,000	648,821	19,338
Donor 2	26		CD4	1	135,000	1,069,588	45,098
			CD8	1	251,000	633,854	28,967
Donor 3	35		CD4	1	200,500	380,253	43,526
				2	231,000	223,661	23,038
			CD8	1	350,500	533,091	47,626
Donor 4	32		CD4	1	560,000	17,494,455	165,184
				2	488,000	12,525,047	113,333
				3	395,000	6,077,772	54,546
				4	100,000	4,353,149	24,582
			CD8	1	510,000	9,219,066	101,096
				2	316,000	10,594,890	61,022
Donor 5	51	Old	CD4	1	495,000	13,765,788	183,873
				2	425,000	5,146,822	64,134
				3	437,000	3,832,144	84,458
				4	255,000	4,773,131	90,459
			CD8	1	509,000	7,369,930	125,339
				2	200,000	2,689,594	64,907
				3	343,000	517,265	20,870
				4	120,000	1,945,472	32,259
Donor 6	88		CD4	1	500,000	779,559	65,515
			CD8	1	60,000	142,639	9,991
Donor 7	51		CD4	1	100,000	28,129	5,033
				2	100,000	37,556	5,455
				3	100,000	41,380	6,762
			CD8	1	105,000	51,463	7,434
Donor 8	82		CD4	1	100,000	46,472	8,318
				2	90,000	38,109	6,694
			CD8	1	12,000	6,013	846
Donor 9	55		CD4	1	100,000	82,691	7,768
				2	100,000	27,215	4,740
			CD8	1	100,000	51,742	5,290
				2	100,000	84,532	7,666
Donor 10	77		CD4	1	254,000	677,587	24,652
			CD4	2	227,000	963,394	20,087
			CD8	1	50,000	942,430	15,033
			CD8	2	105,000	553,867	19,180
Donor 11	53		CD4	1	153,000	401,550	11,668
	53		CD8	1	101,000	242,248	6,857

Donors, replicas, sorted cell counts, and number of extracted T-cell receptor beta CDR3 clonotypes are shown.