Table 1.
Antibodies for FACS analysis of synovial leukocyte populations.
Target | Label | Dilution | Manufacturer | Reference | Concentration |
---|---|---|---|---|---|
anti-CD45 | APC-CY7 | 1:400 | eBioscience | 47-0451-82 | 0.2 mg/ml |
anti- CD11b | PerCP CY5.5 | 1:200 | BioLegend | 45-0112-82 | 0.2 mg/ml |
anti-CD11c | FITC | 1:100 | eBioscience | 11-0114-82 | 0.5 mg/ml |
anti-SiglecF | eFlour660 | 1:100 | eBioscience | 50-1702-80 | 0.2 mg/ml |
anti-CD64 | PE-Cy7 | 1:100 | BioLegend | 139314 | 0.2 mg/ml |
anti-Ly6g | PE Dazzle | 1:800 | BioLegend | 127648 | 0.2 mg/ml |
anti-Ly6c | BV510 | 1:600 | BioLegend | 128033 | 0.2 mg/ml |
anti-MHC Class II | BV711 | 1:800 | Biolegend | 107643 | 0.2 mg/ml |
anti-F4/80 | PE labelled | 1:400 | BioLegend | 123110 | 0.2 mg/ml |
anti-CD45 | APC-Cy7 | 1:400 | eBioscience | 47-0451-82 | 0.2 mg/ml |
anti-CD3 | PE-Cy7 | 1:400 | eBioscience | 25-0031-82 | 0.2 mg/ml |
anti-CD4 | Pacific blue | 1:600 | eBioscience | 116008 | 0.5 mg/ml |
anti-CD19 | PE labelled | 1:400 | eBioscience | 12-0193-82 | 0.2 mg/ml |
anti-CD8 | Texas red | 1:800 | eBioscience | MCD0817 | 0.2 mg/ml |