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. 2018 Aug;92:104–113. doi: 10.1016/j.jaut.2018.05.010

Table 1.

Antibodies for FACS analysis of synovial leukocyte populations.

Target Label Dilution Manufacturer Reference Concentration
anti-CD45 APC-CY7 1:400 eBioscience 47-0451-82 0.2 mg/ml
anti- CD11b PerCP CY5.5 1:200 BioLegend 45-0112-82 0.2 mg/ml
anti-CD11c FITC 1:100 eBioscience 11-0114-82 0.5 mg/ml
anti-SiglecF eFlour660 1:100 eBioscience 50-1702-80 0.2 mg/ml
anti-CD64 PE-Cy7 1:100 BioLegend 139314 0.2 mg/ml
anti-Ly6g PE Dazzle 1:800 BioLegend 127648 0.2 mg/ml
anti-Ly6c BV510 1:600 BioLegend 128033 0.2 mg/ml
anti-MHC Class II BV711 1:800 Biolegend 107643 0.2 mg/ml
anti-F4/80 PE labelled 1:400 BioLegend 123110 0.2 mg/ml
anti-CD45 APC-Cy7 1:400 eBioscience 47-0451-82 0.2 mg/ml
anti-CD3 PE-Cy7 1:400 eBioscience 25-0031-82 0.2 mg/ml
anti-CD4 Pacific blue 1:600 eBioscience 116008 0.5 mg/ml
anti-CD19 PE labelled 1:400 eBioscience 12-0193-82 0.2 mg/ml
anti-CD8 Texas red 1:800 eBioscience MCD0817 0.2 mg/ml