Table 2.
Pisatin production in pea endocarp tissue 24 h after treatment with DNA-specific compounds, capable of DNA intercalation or minor groove localization.
| Agent appl. mg/mL -> | 1.0 | 0.5 | 0.25 | 0.12 | 0.06 | 0.03 | 0.015 |
| Mithramycin | 258.5 | 209.6 | 264.9 | 283.8 | 146.0 | 3.2 | 0.0 |
| Ethidium br. | 18.2 | 43.1 | 130.9 | 104.9 | 97.2 | 131.7 | 131.6 |
| Acrid. orange | 104.3 | 14.9 | 9.7 | 9.8 | 9.0 | 6.0 | 8.0 |
| Chitosan hep. | 50.4 | 95.9 | 8.4 | 25.2 | 19.4 | 7.6 | – |
| Distamycin A | 73.3 | 40.5 | 30.3 | 22.9 | 14.3 | 6.9 | 3.7 |
| Neomycin | 62.1 | 5.2 | 0.0 | 0.0 | 0.0 | 0.1 | 0.0 |
| Daunomycin | 44.5 | 44.3 | 52.1 | 52.2 | 4.7 | 4.9 | 2.1 |
| Spermine | 22.9 | 37.3 | 15.6 | 17.5 | 5.6 | 9.5 | – |
| Hoechst33258 | 24.1 | 14.3 | 17.8 | 8.0 | 0.0 | 13.9 | 0.0 |
| DAPI | 10.5 | 7.5 | 8.9 | 8.4 | 4.7 | 4.3 | 5.5 |
Pisatin (μg/g fresh weight) produced by pea endocarp tissue in 24 h following the application (25 μL/pod half) of the respective concentrations of DNA-specific agents were measured. Pisatin was extracted and analyzed by protocol (Hadwiger and Tanaka, 2017a). Values represent the average of two extractions. Water treated tissues produced no detectible pisatin spectra and were used to develop a baseline of 309 nm absorbance. Average of two replications. The variance in range between replicate values did not exceed 20%.