Table 1.
Comparing effects of LPS and I+T on transcript levels of pro-inflammatory mediators.
| mRNA counts | Fold change with respect to Control | ||
|---|---|---|---|
| Gene | Control | LPS | I+T |
| C1r | 59 ± 40 | 4.15 ± 2.39↑↑ | 5.54 ± 3.35↑↑↑ |
| C5ar1 | 2825 ± 665 | 6.86 ± 1.3↑↑↑*** | 0.25 ± 0.07↓↓↓ |
| Casp1 (ICE) | 475 ± 112 | 2.80 ± 0.72↑↑↑** | 1.55 ± 0.10↑ |
| Ccl3 | 3667 ± 823 | 71.68 ± 16.04↑↑↑*** | 0.52 ± 0.12↓↓ |
| Ifng | 4 ± 3 | 4.17 ± 3.60↑ | 1.50 ± 1.39 |
| Ifngr1 | 5054 ± 717 | 1.71 ± 0.71 | 1.26 ± 0.26 |
| Ifngr2 | 18 ± 7 | 2.11 ± 0.50↑↑↑ | 1.52 ± 0.22↑ |
| Il1b | 1011 ± 607 | 192.60 ± 31.04↑↑↑*** | 1.24 ± 0.33 |
| Il1r1 | 7 ± 3 | 14.79 ± 5.41↑↑↑*** | 1.72 ± 0.48 |
| Il1r2 | 7 ± 4 | 7.79 ± 5.20↑↑↑* | 1.80 ± 0.75 |
| Il6 | 5 ± 4 | 2910.45 ± 649.57↑↑↑*** | 4.17 ± 2.23↑↑↑ |
| Nos2 (iNOS) | 41 ± 42 | 5479.50 ± 1313.28↑↑↑** | 1030.13 ± 125.70↑↑↑ |
| Ptgs2 (COX-2) | 13 ± 10 | 1471.12 ± 710.39↑↑↑*** | 28.66 ± 17.08↑↑↑ |
| Ptk2b (PYK2) | 875 ± 182 | 6.28 ± 0.97↑↑↑ | 11.75 ± 0.43↑↑↑*** |
| Tnf (TNFα) | 325 ± 127 | 8.15 ± 1.60↑↑↑* | 4.20 ± 0.88↑↑↑ |
| Tnfrsf1a (TNFR1) | 657 ± 67 | 3.38 ± 0.77↑↑↑ | 3.49 ± 0.40↑↑↑ |
| Tnfrsf1b (TNFR2) | 1126 ± 139 | 22.91 ± 4.37↑↑↑*** | 2.71 ± 0.40↑↑↑ |
Rat microglia were stimulated for 24 h with LPS or a combination of IFNγ + TNFα (I+T). For clarity, protein names are included in parentheses for some common genes. Basal mRNA levels in unstimulated (control) cells are expressed as mean counts/200 ng RNA sample ± SD (n = 6–7 individual microglia cultures). Fold changes were used to assess effects of LPS or I+T on each gene. Arrows indicate statistically significant increases (↑) or decreases (↓) relative to control cells. Asterisks (∗) indicate differences between LPS and I+T. One symbol of either type indicates p < 0.05; two, p < 0.01; three, p < 0.001.