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. 2018 Jul 24;9:1016. doi: 10.3389/fpls.2018.01016

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Copyright © 2018 Li, Wei, Ahammed, Zhang, Li, Yan, Zhang and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-course analysis of theanine biosynthetic genes as influenced by the SHT in tea leaves. Two years-old tea [Camellia sinensis (L.) O. Kuntze cv. Longjing 43] seedlings were exposed to SHT (35°C) for 5 days in temperature-controlled chambers. One bud and two leaves under the bud were sampled at the indicated time-points to analyze transcript abundance of ARGININE DECARBOXYLASE (CsADC), GLUTAMINE SYNTHETASE (CsGS), GLUTAMATE SYNTHASE (CsGOGAT), and THEANINE SYNTHASE (CsTS) using real-time qPCR. Data are presented as the mean of three biological replicates (±SD). Means denoted by the same letter at the same time point do not significantly differ at P < 0.05 according to Tukey’s test.