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. 2018 Jul 24;9:1016. doi: 10.3389/fpls.2018.01016

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Copyright © 2018 Li, Wei, Ahammed, Zhang, Li, Yan, Zhang and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-course effect of SHT on the activity of key enzymes involved in theanine biosynthesis in tea leaves. Two years-old tea [C. sinensis (L.) O. Kuntze cv. Longjing 43] seedlings were exposed to SHT (SHT, 35°C) for 5 days in temperature-controlled chambers. Tea seedlings were pretreated with 0.2 ppm 24-epibrassinolide until surface run-off, 12 h prior to imposition of the SHT treatment. One bud and two leaves under the bud were sampled at the indicated time-points to analyze glutamine synthetase (GS) and glutamate synthase (GOGAT) activity. Data are presented as the mean of six replicates (±SD). Means denoted by the same letter at the same time point do not significantly differ at P < 0.05 according to Tukey’s test.