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A) An overview of light scattering by a molecule. Light scattered by a molecule contains elastic (λs = λi) and inelastic (λs ≠ λi) components. B) A schematic representation of the relationship between wavelength shift of Raman scattering and vibrational energy of a molecule in a scattering spectrum. C) Raman spectra of biomolecules. λi: wavelength of incident light. λs, λs1, λs2, λs3, λs4: wavelengths of scattering light. a. u., arbitrary units.
