Figure 6. Real-time monitoring of TEER.
HUVEC were seeded (2 × 105 cells/well) in gelatin A-coated golden ECIS ‘8W10E+’ well arrays. After formation of a confluent HUVEC monolayer, cells were treated with 100 ng/ml TNF-α in the presence or absence of 10 µM of either RES, RES-LA, or SB-3CT. Real-time TEER measurements were done at and 8-s frequency. Results were analyzed using ECIS software. (A) Represents untreated, negative controls (0.1% NADES or DMSO), and TNF-α groups. (B) Represents RES, RES-LA, or SB-3CT groups.