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. 2018 Jul 6;3(7):1222–1232. doi: 10.1021/acssensors.8b00340

Figure 4.

Figure 4

(A) Preparation of disc-based bioassay and signal amplification using gold/silver staining. (1) UV/ozone activation to generate carboxylic acid groups on CD surface; (2) immobilization of amino-tethered DNA probe strands via amide coupling; (3) hybridization with biotinylated DNA target strands; (4) binding of gold nanoparticle–streptavidin conjugates; (5) reductive precipitation of silver particles for signal enhancement. (B) Digital reading of bioassay using CD drive. The biomolecule/nanoparticle conjugates block the reading laser and generate errors. (C) Optical image of DNA microarray formed on a regular CD-R according to the above surface reaction and signal amplification. Reprinted with permission from ref (37). Copyright 2008 American Chemical Society.