Table 1.

Primers used for polymerase chain reaction analysis.

Primer	Sequence (5′–3′)	PCR conditions	Size of PCR product	Reference
HEL-F	AAC GAT GAA GCT TCT AGC TTG CTA	Initial denaturation at 94 °C for 10 min; 35 cycles of 94 °C for 30 s, 56 °C for 1 min and 72 °C for 1 min; final extension of 72 °C for 10 min	399bp	12
HEL-R	GTG CTT ATT CST NAG ATA CCG TCA T
glmM-F	GGA TAA GCT TTT AGG GGT GTT AGG GG		294pb	13
glmM-R	GCT TAC TTT CTA ACA CTA ACG CGC	.
cagA-F1	GAT AAC AGG CAA GCT TTT GAG G	Initial denaturation at 94 °C for 10 min; 40 cycles of 94 °C for 30 s, 58 °C for 30 s and 72 °C for 1 min; final extension of 72 °C for 10 min	349pb	14,15
cagA-B1	CTG CAA AAG ATT GTT TGG CAG A
VA1-F	ATG GAA ATA CAA CAA ACA CAC		259pb	14
VA1-R	CTG CTT G AATGC GCC AAAC
VA1-F	ATG GAA ATA CAA CAA ACA CAC	Initial denaturation at 94 °C for 10 min; 35 cycles of 94 °C for 30 s, 60 °C for 30 s and 72 °C for 30 s; final extension of 72 °C for 10 min.	286pb	14
VA1-R	CTG CTT G AATGC GCC AAAC
VAG-F	CAA TCT GTC CAA TCA AGC GAG		570pb	14
VAG-R	GCG TCA AAT AAT T CCA AGG
VAG-F	CAA TCT GTC CAA TCA AGC GAG		645pb	14
VAG-R	GCG TCA AAT AAT T CCA AGG			14