Fig. 5.

RLL peaks are enriched for replication origins while RLG peaks are depleted. a Heatmap of enrichment or depletion of RLG and RLL peaks over specific chromatin features. Color codes and description are as Fig. 4a. b Distance between RLG and RLL peaks and replication origins compared to matched controls. Statistical significance was measured by Wilcoxon test. c Distance between replication origins and all R-loop peaks ranked by the strength of R-loop changes (color-coded). d SNS-seq signal plotted over promoter and terminal regions for RLL and RLG loci as well as matched controls. Data is shown as median with standard error (shaded). e SNS-seq replication signal of R-loop peaks ranked by the strength of R-loop gains and losses (color-coded). f Replication timing analysis for RLL, RLG and matched peaks according to phases of the cell cycle based on Repli-seq data. All comparisons to matched peaks are significant (p < 0.008, Monte-Carlo) except when indicated (NS)