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Killing activity of inhibitory concentrations of N-SCN on M. smegmatis. After a 24-h treatment with an inhibitory concentration of N-SCN (8 × MIC), the complemented knock-down strain MsPptrtopoI strain (gray bars) was killed at a lower rate than its non-complemented parental strain (white bars); this difference was significant (P < 0.05). The mean ± standard error of the mean from at least three independent replicates is shown.
